Recombinant TNFRSF10B antibody (Extracellular Domain)

Catalog No. ABIN7072462

This Rabbit Chimeric antibody specifically detects TNFRSF10B in WB, FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant TNFRSF10B antibody (Extracellular Domain) (ABIN7072462)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This TNFRSF10B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Clone: TRA-8

Product Details anti-TNFRSF10B Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-DR5 [TRA-8], Rabbit IgG, kappa

Specificity:

The antibody is specific to human DR5 and does not bind other TRAIL receptors, other members of the TNF-receptor superfamily, or the murine homolog of DR5.

Application Notes original Clone: The antibody has been characterised using Western blot and ELISA. It induces apoptosis in primary hepatocellular carcinoma cells and established liver cancer cell lines, but not normal human hepatocytes (Ichikawa et al., 2001). The antibody has been reported to function in flow cytometry to detect cell-surface expression of DR5 on Cos-7 cells transfected with full-length human DR5. This antibody has been demonstrated to have therapeutic benefits in the treatment of cancer in vivo and ex vivo (Buchsbaum et al., 2003, PMID: 14506165) (Straughn Jr et al., 2006, PMID: 16271751) (Estes et al., 2007, PMID: 17303227).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The antibody was raised by immunizing BALB/c mice with a fusion protein containing the extracellular portion of human DR5 and the Fc portion of human IgG1.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: DR5

Background: TRAILR2, TNFRSF10B, TRAIL receptor 2, tumor necrosis factor receptor superfamily member 10B

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity