Recombinant Integrin beta 2 antibody

Catalog No. ABIN7072534

This Rabbit Chimeric antibody specifically detects Integrin beta 2 in FACS, IP, ELISA, IF and RIA. It exhibits reactivity toward Human and Rhesus Monkey.

Quick Overview for Recombinant Integrin beta 2 antibody (ABIN7072534)

Target: Integrin beta 2 (ITGB2)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rhesus Monkey

Host: Rabbit

Clonality: Chimeric

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Radioimmunoassay (RIA)

Clone: 1B4

Product Details anti-Integrin beta 2 Antibody

Purpose: Anti-CD18 [1B4], Rabbit IgG, kappa

Specificity: Specific to the beta chain of the leukocyte integrin beta-2 family. This is found in LFA-1, Mac-1 and p150.95 among others. Binds an epitope distinct from the C3bi binding site. Has been shown to recognise both the human and Rhesus monkey antigen.

Cross-Reactivity: Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.

Isotype: IgG kappa

Application Details

Application Notes: The main application 1B4 has been proposed for is its anti-inflammatory activity. It has been shown to work as a Fab fragment prepared by papain digestion (Wright et al, 1983). Its binding to antigen was confirmed by RIA (Van Voorhis et al, 1983). 1B4 has been shown to work in Rhesus monkeys, and has reduced immunogenicity once humanized. 1B4 is also compatible with immunofluorescence which has been used to demonstrate that the fine specificity of the combining sites was not altered by humanization (Singer et al, 1993). It has been shown to prevent polymorphonuclear (PMN)-dpepndent pathology in animal models of hemorrhagic shock, dermal inflammation, reperfusion injury, IBD and meningitis (Vedder et al, 1988, Vedder et al, 1990, Mileski et al, 1990, Carlos et al, 1990, Tuomanen et al, 1989, Arfors et al, 1987, Wallace et al, 1991, Wallace et al, 1992). The glycosylation patterns of 1B4 have also been studied in detail using NMR, ES-MS, HPAEC-PAD mapping and glycosyl composition analysis (Yu Ip et al, 1994).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18

Background: Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, ITGB2, MFI7, IB4, m1B4, mIB4

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation