Recombinant ErbB2/Her2 antibody (Extracellular Domain)

Catalog No. ABIN7072537

This Rabbit Chimeric antibody specifically detects ErbB2/Her2 in IF, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant ErbB2/Her2 antibody (Extracellular Domain) (ABIN7072537)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: FWP51

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-c-erbB-2 [FWP51], Rabbit IgG, kappa

Specificity:

FWP51 binds to the extracellular domain of c-erbB-2.

Application Notes original Clone: Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: 20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: c-erbB-2

Background: CD340, Receptor tyrosine-protein kinase erbB-2, Neu, HER2, MLN19, Metastatic lymph node gene 19 protein, p185erbB2

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development