Recombinant CD4 antibody

Catalog No. ABIN7072643

The Mouse Monoclonal anti-CD4 antibody is suitable to detect CD4 in samples from Human. It has been validated for FACS, IHC and IF.

Quick Overview for Recombinant CD4 antibody (ABIN7072643)

Target: CD4

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: EDU-2 (B486)

Product Details anti-CD4 Antibody

Purpose: Anti-CD4 [EDU-2 (B486)], Mouse IgG2a, kappa

Specificity: EDU-2 (B486) recognizes CD4, which is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with stimulated human leukocytes.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody is recommended for the detection of CD4 via immunofluorescence and flow cytometry. For instace, one group utilized EDU-2 antibody to identify CD4-posotive cells via a modified flow cytometric assay in order to develop efficient and cost-effective method for HIV infection monitoring (Barbesti et al., 2005). Furthermore, EDU-2 antibody was also used to differentiate between different subsets of dendritic cells (Maniecki et al., 2006).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Leu3/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events