Recombinant Integrin beta 2 antibody

Catalog No. ABIN7072727

The Rabbit Chimeric anti-Integrin beta 2 antibody has been validated for WB, ELISA, BR and InhA. It is suitable to detect Integrin beta 2 in samples from Human.

Quick Overview for Recombinant Integrin beta 2 antibody (ABIN7072727)

Target: Integrin beta 2 (ITGB2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: 60-3

Product Details anti-Integrin beta 2 Antibody

Purpose: Anti-CD18 [60.3], Rabbit IgG, kappa

Specificity: This antibody binds the CD18 component of the β-2 integrin family which is expressed by leukocytes and neutrophils.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is capable of inhibiting several cell mediated immune functions. It inhibits proliferative responses of T cells stimulated by soluble antigens, mitogens, or allogeneic cells in the initiation of a culture, but not when added after 48 hours. It successfully blocks the lytic activity of cytotoxic T cells and natural killer cells and the migration of neutrophilic granulocytes (PMID: 6644024). This antibody inhibits phagocytosis by reaction with the C3bi receptor, and stimulates phagocytosis by reverse opsonization (PMID: 3880787). This antibody inhibits aggregation of neutrophils in response phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine in a dose dependent manner (PMID: 3995185). 60.3 also inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo (PMID: 3539230). 60.3 successfully inhibits neutrophil function in vivo and has the potential to be used as an anti-inflammatory agent (PMID: 3693901). This antibody has application in blocking neutrophil mediated organ damage which occurs after myocardial infarct or hemorrhagic shock. Humanized version of this antibody binds CD18 with a 4-fold less affinity than its parent murine version (PMID: 7937713).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18

Background: ITGB2, MFI7, Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, β2-subunit of leukocyte integrins

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation