Cullin 2 antibody (AA 365-745) (HRP)

Catalog No. ABIN705275

The Rabbit Polyclonal anti-Cullin 2 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect Cullin 2 in samples from Human.

Quick Overview for Cullin 2 antibody (AA 365-745) (HRP) (ABIN705275)

Target: Cullin 2 (CUL2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 2 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cullin 2 Antibody (HRP)

Binding Specificity: AA 365-745

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cullin 2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: Cullin 2

Background:

Synonyms: Cullin2, Cullin-2, CUL 2, MGC131970, CUL2_HUMAN.

Background: Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Gene ID: 8453

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome