MGMT antibody (AA 1-100) (Biotin)

Catalog No. ABIN7091485

The Rabbit Polyclonal anti-MGMT antibody has been validated for WB and ELISA. It is suitable to detect MGMT in samples from Rat.

Quick Overview for MGMT antibody (AA 1-100) (Biotin) (ABIN7091485)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-MGMT Antibody (Biotin)

Binding Specificity: AA 1-100

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse MGMT

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background:

Synonyms: 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT: O-6 methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6 methylguanine DNA alkyltransferase, O-6-methylguanine-DNA methyltransferase, O6 methylguanine DNA methyltransferase, MGMT_HUMAN.

Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene ID: 17314

UniProt: P26187

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus