DNA2 antibody

Catalog No. ABIN7113558

This anti-DNA2 antibody is a Rabbit Polyclonal antibody detecting DNA2 in ELISA, IHC and WB. Suitable for Human, Mouse and Rat.

Quick Overview for DNA2 antibody (ABIN7113558)

Target: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-DNA2 Antibody

Purpose: DNA2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: DNA replication helicase 2 homolog

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for DNA2

Target: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Alternative Name: DNA2

Background:

Synonyms: DNA replication ATP-dependent helicase/nuclease DNA2 (hDNA2)|DNA replication ATP-dependent helicase-like homolog|DNA2|DNA2L|KIAA0083

Background: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Molecular Weight: 127 kDa

Gene ID: 1763

UniProt: P51530

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA