NEIL1 antibody

Catalog No. ABIN7113799

This Rabbit Polyclonal antibody specifically detects NEIL1 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NEIL1 antibody (ABIN7113799)

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEIL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEIL1 Antibody

Purpose: NEIL1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: nei endonuclease VIII-like 1(E. coli)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Alternative Name: NEIL1

Background:

Synonyms: Endonuclease 8-like 1|DNA glycosylase/AP lyase Neil1|DNA-(apurinic or apyrimidinic site) lyase Neil1|Endonuclease VIII-like 1|FPG1|Nei homolog 1 (NEH1)|Nei-like protein 1|NEIL1

Background: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine(Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP(apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'-and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine(5hmC), suggesting that it acts as a specific reader of 5hmC.

Molecular Weight: 44 kDa

Gene ID: 79661

UniProt: Q96FI4

Pathways: DNA Damage Repair