GOT1 antibody (AA 1-413)

Catalog No. ABIN7114620

The Mouse Monoclonal anti-GOT1 antibody (Clone 2F1) (ABIN7114620) specifically detects GOT1 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for GOT1 antibody (AA 1-413) (ABIN7114620)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 2F1

Product Details anti-GOT1 Antibody

Binding Specificity: AA 1-413

Purpose: GOT1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-413aa

Immunogen: glutamic-oxaloacetic transaminase 1, soluble(aspartate aminotransferase 1)

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1

Background:

Synonyms: Aspartate aminotransferase, cytoplasmic (cAspAT)|Cysteine aminotransferase, cytoplasmic|Cysteine transaminase, cytoplasmic (cCAT)|Glutamate oxaloacetate transaminase 1|Transaminase A|GOT1

Background: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase(3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Molecular Weight: 43 kDa

Gene ID: 2805

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process