GRP78 antibody

Catalog No. ABIN7114888

This Mouse Monoclonal antibody specifically detects GRP78 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GRP78 antibody (ABIN7114888)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 3F8

Product Details anti-GRP78 Antibody

Purpose: HSPA5 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: Endoplasmic reticulum chaperone BiP

Isotype: IgG1

Application Details

Application Notes: WB: 1:2000-1:20000, IHC: 1:50-1:200, IF: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5

Background:

Synonyms: Endoplasmic reticulum chaperone BiP|78 kDa glucose-regulated protein (GRP-78)|Binding-immunoglobulin protein (BiP)|Heat shock protein 70 family protein 5 (HSP70 family protein 5)|Heat shock protein family A member 5|Immunoglobulin heavy chain-binding protein|HSPA5|GRP78

Background: GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Molecular Weight: 70-78 kDa

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling