IFITM3 antibody (AA 1-133)

Catalog No. ABIN7115388

The Mouse Monoclonal anti-IFITM3 antibody (Clone 5H4) (ABIN7115388) specifically detects IFITM3 in WB, ELISA, IF, IHC and FACS. The antibody is reactive with Human samples.

Quick Overview for IFITM3 antibody (AA 1-133) (ABIN7115388)

Target: IFITM3 (Interferon-Induced Transmembrane Protein 3 (IFITM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFITM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5H4

Product Details anti-IFITM3 Antibody

Binding Specificity: AA 1-133

Purpose: IFITM3 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-133aa

Immunogen: IFITM2/3 fusion protein

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for IFITM3

Target: IFITM3 (Interferon-Induced Transmembrane Protein 3 (IFITM3))

Alternative Name: IFITM3

Background:

Synonyms: Interferon-induced transmembrane protein 3|Dispanin subfamily A member 2b (DSPA2b)|Interferon-inducible protein 1-8U|IFITM3

Background: IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus(SARS-CoV), Marburg virus(MARV) and Ebola virus(EBOV), Dengue virus(DNV), West Nile virus(WNV), human immunodeficiency virus type 1(HIV-1) and vesicular stomatitis virus(VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase(v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.This antibody recognizes both IFITM2 and IFITM3.

Molecular Weight: 14 kDa

Gene ID: 10410

UniProt: Q01628