MBD3 antibody

Catalog No. ABIN7116242

The Rabbit Polyclonal anti-MBD3 antibody is suitable to detect MBD3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and IP.

Quick Overview for MBD3 antibody (ABIN7116242)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-MBD3 Antibody

Purpose: MBD3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: methyl-CpG binding domain protein 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background:

Synonyms: Methyl-CpG-binding domain protein 3|Methyl-CpG-binding protein MBD3|MBD3

Background: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself(PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides(in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides(PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Molecular Weight: 33 kDa

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding