Nischarin antibody

Catalog No. ABIN7116889

This Rabbit Polyclonal antibody specifically detects Nischarin in WB, IP and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Nischarin antibody (ABIN7116889)

Target: Nischarin (NISCH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nischarin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-Nischarin Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: nischarin

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for Nischarin

Target: Nischarin (NISCH)

Alternative Name: NISCH

Background: Synonyms:IRAS, KIAA0975 Background:Acts either as the functional imidazoline-1 receptor(I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla(RVLM) neurons that exhibited rilmenidine-evoked hypotension(By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons(By similarity). Acts as a modulator of Rac-regulated signal transduction pathways(By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity(By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation(By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation(By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells(By similarity). Inhibits lamellipodia formation, when overexpressed(By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.

Molecular Weight: 190-200 kDa

Gene ID: 11188

UniProt: Q9Y2I1