Nischarin antibody (AA 1100-1150)

Catalog No. ABIN7451170

Product Details anti-Nischarin Antibody

Target: Nischarin (NISCH)

Binding Specificity: AA 1100-1150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nischarin antibody is un-conjugated

Application: Immunoprecipitation (IP)

Purpose: Rabbit anti-NISCH Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 1100 and 1150

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451170 has not performed satisfactorily when used for WB of NISCH in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NISCH.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Nischarin

Target: Nischarin (NISCH)

Alternative Name: NISCH (NISCH Products)

Synonyms: NISCH antibody, IRAS antibody, 1200007D05Rik antibody, 3202002H23Rik antibody, AW494485 antibody, I-1 antibody, IR1 antibody, hIRAS antibody, nischarin antibody, nischarin S homeolog antibody, NISCH antibody, Cyan7822_6841 antibody, nisch.S antibody, nisch antibody, Nisch antibody

Background: Background: Nischarin (NISCH) is a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of NISCH was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis, possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation[taken from NCBI Entrez Gene (Gene ID: 11188)].

Gene ID: 11188

UniProt: Q9Y2I1