PSME4 antibody

Catalog No. ABIN7117899

This anti-PSME4 antibody is a Rabbit Polyclonal antibody detecting PSME4 in WB, ELISA, IHC, IP and IF. Suitable for Human, Mouse and Rat.

Quick Overview for PSME4 antibody (ABIN7117899)

Target: PSME4 (Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSME4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-PSME4 Antibody

Purpose: PSME4 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: proteasome(prosome, macropain) activator subunit 4

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000 , IHC: 1:50-1:500, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PSME4

Target: PSME4 (Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4))

Alternative Name: PSME4

Background:

Synonyms: Proteasome activator complex subunit 4|Proteasome activator PA200|PSME4|KIAA0077

Background: Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP-and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like(BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.

Molecular Weight: 140 kDa

Gene ID: 23198

UniProt: Q14997

Pathways: DNA Replication, Synthesis of DNA