TNF alpha antibody (AA 1-233)

Catalog No. ABIN7120393

This Mouse Monoclonal antibody specifically detects TNF alpha in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TNF alpha antibody (AA 1-233) (ABIN7120393)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 2A9

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 1-233

Purpose: TNF alpha antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-233aa

Immunogen: tumor necrosis factor(TNF superfamily, member 2)

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background:

Synonyms: Tumor necrosis factor|Cachectin|TNF-alpha|Tumor necrosis factor ligand superfamily member 2 (TNF-a)|Tumor necrosis factor, membrane form Alternative names: N-terminal fragment (NTF)|Intracellular domain 1 (ICD1)|Intracellular domain 2 (ICD2)|C-domain 1|C-domain 2|Tumor necrosis factor, soluble form|TNF|TNFA|TNFSF2

Background: TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme(TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Molecular Weight: 22 kDa

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome