Vimentin antibody

Catalog No. ABIN7120771

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Vimentin antibody (ABIN7120771)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 2G10

Product Details anti-Vimentin Antibody

Purpose: VIM antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vimentin

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-1:10000, IHC: 1:200-1:2000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Background:

Synonyms: Vimentin|VIM

Background: Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA, 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Molecular Weight: 55 kDa

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis