Recombinant CDKN2A antibody

Catalog No. ABIN7504172

Product Details anti-CDKN2A Antibody

Target: CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4) (CDKN2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: P16INK4a is a tumor suppressor protein. It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia. Expression of p16INK4a (p16 positive) is highly correlated with human papilloma virus (HPV) infection in head and neck squamous cell carcinomas (HNSCC). p16 status is an important prognostic indicator in HNSCC and the p16 positive/HPV16 negative group is likely a distinct subgroup lacking any HPV genotype.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified recombinant prokaryotic full-length human p16INK4a protein

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: Human cervical squamous cell carcinoma.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CDKN2A

Target: CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4) (CDKN2A))

Alternative Name: ARF (CDKN2A Products)

Synonyms: Arf antibody, p16 antibody, MTS1 antibody, INK4A antibody, p19ARF antibody, p16Cdkn2a antibody, ARF antibody, CDK4I antibody, CDKN2 antibody, CMM2 antibody, INK4 antibody, MLM antibody, MTS-1 antibody, P14 antibody, P14ARF antibody, P16 antibody, P16-INK4A antibody, P16INK4 antibody, P16INK4A antibody, P19 antibody, P19ARF antibody, TP16 antibody, ARF-INK4a antibody, INK4a-ARF antibody, Ink4a/Arf antibody, Pctr1 antibody, p16(INK4a) antibody, p16INK4a antibody, p19 antibody, cyclin-dependent kinase inhibitor 2A antibody, cyclin dependent kinase inhibitor 2A antibody, Cdkn2a antibody, LOC100731972 antibody, LOC100340625 antibody, CDKN2A antibody

Background: CDK4 inhibitor p16 INK4, CDK4I, CDKN2A, Cell cycle negative regulator beta, CMM2, Cyclin dependent kinase 4 inhibitor A, Melanoma p16 inhibits CDK4, MLM, MTS1, Multiple tumor suppressor 1, p14, p16, p19, P19ARF, TP16,P16INK4a
Cellular localisation: Nucleus. Cytoplasm.

Molecular Weight: 14kDa

Gene ID: 1029, 512599

UniProt: P42771

Pathways: Mitotic G1-G1/S Phases, Stem Cell Maintenance, Positive Regulation of Endopeptidase Activity, Autophagy, Positive Regulation of Response to DNA Damage Stimulus