Recombinant CDKN1B antibody

Catalog No. ABIN7126172

This Rabbit Monoclonal antibody specifically detects CDKN1B in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Hamster and Guinea Pig.

Quick Overview for Recombinant CDKN1B antibody (ABIN7126172)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Rabbit, Hamster, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: KIP1-1355R

Product Details anti-CDKN1B Antibody

Purpose: Recombinant p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody

Specificity: This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- 79oΩ 79oΩ induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CDKN1B protein

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells. Human tonsil, breast, cervical or colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B

Background:

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Breast, Cervical or Colon Carcinoma., NIH/3T3, HeLa or MCF7, A431 cells. Human tonsil, Human Spleen, Skeletal Muscle

Molecular Weight: 25-26kDa

Gene ID: 1027, 238990

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy