Angiotensin I Converting Enzyme 1 antibody (AA 652-788)

Catalog No. ABIN7126286

This Mouse Monoclonal antibody specifically detects Angiotensin I Converting Enzyme 1 in WB, ELISA, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (AA 652-788) (ABIN7126286)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Binding Specificity: AA 652-788

Specificity: This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 652-788) of human ACE/CD143 protein

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE

Background: ACE, ACE1, angiotensin 1 converting enzyme 1, carboxycathepsin, CD143, CD143 antigen, DCP, DCP1, dipeptidyl carboxypeptidase 1, Kininase II, MVCD3, peptidase P, testicular ECA,Angiotensin I Converting Enzyme (ACE) / CD143
Cellular localisation: Cell Surface

Molecular Weight: 150kDa

Gene ID: 1636, 298469

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration