GLIS3 antibody

Catalog No. ABIN7126297

Product Details anti-GLIS3 Antibody

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLIS3 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Coating (Coat), Immunoprecipitation (IP)

Specificity: GLIS3 is a member of the GLI similar zinc finger protein family, and encodes a nuclear protein with five C2H2 type zinc finger domains. It functions as both an activator and repressor of transcription, and is specifically involved in the development of pancreatic beta cells, thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only two have been determined.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GLIS3 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.

Positive_Control: A549 or U-2 OS cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GLIS3

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Alternative Name: GLIS3 (GLIS3 Products)

Synonyms: ZNF515 antibody, 4833409N03Rik antibody, E230012L24 antibody, E330013K21Rik antibody, GLIS3 antibody, wu:fa55a05 antibody, zgc:158249 antibody, glis3 antibody, pc antibody, RGD1565505 antibody, GLIS family zinc finger 3 antibody, GLIS3 antibody, Glis3 antibody, glis3 antibody

Background: FLJ38999, FLJ90578, GLI similar 3, GLI SIMILAR PROTEIN 3, GLIS family zinc finger 3, MGC33662, OK/KNS-cl.4, Zinc finger protein 515, Zinc finger protein GLIS3, ZNF515,GLIS family zinc finger 3 (GLIS3) / ZNF515
Cellular localisation: Nucleus.

Molecular Weight: 84kDa

Gene ID: 169792, 162125

UniProt: Q8NEA6