Endoglin antibody (AA 74-251)

Catalog No. ABIN7126333

The Mouse Monoclonal anti-Endoglin antibody (Clone ENG-1326) (ABIN7126333) specifically detects Endoglin in WB, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Endoglin antibody (AA 74-251) (ABIN7126333)

Target: Endoglin (ENG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ENG-1326

Product Details anti-Endoglin Antibody

Binding Specificity: AA 74-251

Purpose: Endoglin / CD105 (Angiogenesis Marker) Antibody

Specificity: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Endoglin protein fragment (aa74-251)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), ,Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A431 and HL-60 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: ENG

Background:

CD105, END, Endoglin, Eng, HHT1, S-endoglin

A431 and HL-60 cells., HeLa

Molecular Weight: 84kDa

Gene ID: 2022, 76753

UniProt: P17813