Endoglin antibody

Catalog No. ABIN7126335

The Mouse Monoclonal anti-Endoglin antibody is suitable to detect Endoglin in samples from Human, Rat, Pig, Horse and Monkey. It has been validated for FACS, ELISA, IF and Func.

Quick Overview for Endoglin antibody (ABIN7126335)

Target: Endoglin (ENG)

Reactivity: Human, Rat, Pig, Horse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Functional Studies (Func)

Clone: ENG-1621

Product Details anti-Endoglin Antibody

Purpose: Endoglin / CD105 (Angiogenesis Marker) Antibody

Specificity: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Cross-Reactivity (Details): Human, Monkey, Horse, Pig and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant extracellular fragment of human Endoglin protein exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Functional Assays (order antibody without BSA and azide), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (2-4 μg/mL) Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A431 or HL-60 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8È105ü79oΩC. Antibody without azide - store at -20 to -80È105ü79oΩC. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: ENG

Background:

CD105, END, Endoglin, Eng, HHT1, S-endoglin

A431 or HL-60 cells., HeLa

Molecular Weight: 80kDa (Monomer), 160kDa (Homodimer)

Gene ID: 2022, 76753

UniProt: P17813