Fibronectin 1 antibody (AA 467-595)

Catalog No. ABIN7126420

The Mouse Monoclonal anti-Fibronectin 1 antibody (Clone FN1-3029) (ABIN7126420) specifically detects Fibronectin 1 in ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for Fibronectin 1 antibody (AA 467-595) (ABIN7126420)

Target: Fibronectin 1 (FN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin 1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: FN1-3029

Product Details anti-Fibronectin 1 Antibody

Binding Specificity: AA 467-595

Purpose: Fibronectin Antibody

Specificity: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 467-595) of human fibronectin protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SW156 cells. Human kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1

Background:

Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

SW156 cells. Human kidney.

Molecular Weight: 220kDa (monomer), 440kDa (dimer)

Gene ID: 2335, 203717

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy