CNOT10 antibody

Catalog No. ABIN7126458

The Mouse Monoclonal anti-CNOT10 antibody is suitable to detect CNOT10 in samples from Human. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for CNOT10 antibody (ABIN7126458)

Target: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNOT10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-CNOT10-1D5

Product Details anti-CNOT10 Antibody

Purpose: CCR4-NOT transcription complex, subunit 10 (CNOT10) Antibody

Specificity: CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CNOT10 protein

Isotype: IgG2c

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CNOT10

Target: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Alternative Name: CNOT10

Background:

FLJ12890, FLJ13165

HeLa or HepG2 cells.

Molecular Weight: 82.31kDa

Gene ID: 25904, 444851

UniProt: Q9H9A5