APP antibody (AA 578-680)

Catalog No. ABIN7126553

This Mouse Monoclonal antibody specifically detects APP in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for APP antibody (AA 578-680) (ABIN7126553)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: APP-3345

Product Details anti-APP Antibody

Binding Specificity: AA 578-680

Purpose: beta Amyloid Antibody

Specificity: Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the æ-Amyloid and Amyloid A4 proteins, which are present in human platelets. Amyloid deposition is associated with type II diabetes, Down syndrome and a variety of neurological disorders, including Alzheimer s disease. The Amyloid precursor protein (APP) undergoes alternative splicing, resulting in several isoforms. Proteolytic cleavage of APP leads to the formation of the 4 kDa Amyloid æ/A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from rabbit anti-serum by Protein A.

Immunogen: Recombinant fragment (around aa578-680) of human Amyloid Beta protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Alzheimer's disease brain. Amyloidosis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background:

CVAP; AAA; AICD-50; PN2; 50; Beta-APP42; AID; S-APP-alpha; 57; AD1; PN-II; Beta-APP40; 42; 40; APPI; Alzheimer disease amyloid protein; PreA4; ABETA; Amyloid intracellular domain 50; 57; 59 AICD-59; S-APP-beta; APP; AICD-57; ABPP; Protease nexin-II; Cerebral vascular amyloid peptide

Alzheimer's disease brain. Amyloidosis.

Molecular Weight: 4-46kDa (•æ-Amyloid), 100-125kDa (Amyloid A4)

Gene ID: 351, 434980

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour