ITGB1 antibody

Catalog No. ABIN7126605

The Mouse Monoclonal anti-ITGB1 antibody is suitable to detect ITGB1 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for ITGB1 antibody (ABIN7126605)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: ITGB1-3613

Product Details anti-ITGB1 Antibody

Purpose: CD29 (Stem Cell Marker) Antibody

Specificity: Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin.

Cross-Reactivity (Details): Human. Does not react with Mouse or Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HT1080 whole cell lysate. HeLa cell line.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1

Background:

Fibrinogen Receptor beta subunit, FNRB, Glycoprotein IIa (GPIIA), Integrin beta-1 (ITGB1), integrin VLA-4 beta subunit, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLA-4 subunit beta, VLA-BETA, VLAB

HT1080 whole cell lysate. HeLa cell line. Human Kidney.

Molecular Weight: 110kDa (non-reduced) & 130kDa (reduced)

Gene ID: 3688, 643813

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome