Recombinant EpCAM antibody (Cytoplasmic Domain)

Catalog No. ABIN7126663

The Rabbit Monoclonal anti-EpCAM antibody (Clone EGP40-7035R) (ABIN7126663) specifically detects EpCAM in IHC. The antibody is reactive with Human, Dog and Cat samples.

Quick Overview for Recombinant EpCAM antibody (Cytoplasmic Domain) (ABIN7126663)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog, Cat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: EGP40-7035R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 289-314, Cytoplasmic Domain

Purpose: Recombinant EpCAM / CD326 (Cytoplasmic Domain) (Epithelial Marker) Antibody

Specificity: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Cross-Reactivity (Details): Human.Dog. Cat.

Purification: 200ug/ml of Ab purified by Protein A.

Immunogen: Recombinant fragment from cytoplasmic domain (around aa 289-314) of human EpCAM (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HT29 cells. Human colon or breast.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: TACSTD1

Background:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, ESA, Epithelial Glycoprotein 314, KSA, TROP1, Epithelial Cell Adhesion Molecule, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

HT29 cells. Human colon or breast., MCF-7

Molecular Weight: 40-43kDa

Gene ID: 4072, 542050

UniProt: P16422