Recombinant LEF1 antibody (AA 100-200)

Catalog No. ABIN7126771

The Mouse Monoclonal anti-LEF1 antibody (Clone rLEF1-6906) (ABIN7126771) specifically detects LEF1 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant LEF1 antibody (AA 100-200) (ABIN7126771)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RLEF1-6906

Product Details anti-LEF1 Antibody

Binding Specificity: AA 100-200

Purpose: Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody

Specificity: Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil and thymus.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background:

Lymphoid enhancer-binding factor 1 (LEF1), T cell specific transcription factor 1 alpha (TCF1a), TCF1 alpha, TCF10, TCF7L3, Transcription factor T cell specific 1 alpha

Human tonsil and thymus.

Molecular Weight: 44kDa

Gene ID: 51176, 726506

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding