Recombinant ZAP70 antibody (AA 247-382)

Catalog No. ABIN7127063

The Rabbit Monoclonal anti-ZAP70 antibody (Clone ZAP70-6492R) (ABIN7127063) specifically detects ZAP70 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant ZAP70 antibody (AA 247-382) (ABIN7127063)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: ZAP70-6492R

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 247-382

Purpose: Recombinant ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody

Specificity: Zeta-associated protein-70 (ZAP-70) is a member of the Syk family of tyrosine kinases, a group of proteins that attach to the zeta chain components of T-cell receptors to signal downstream events involved in the regulation of cell function, proliferation, and death. Research suggests that the ZAP-70 protein may also play an important role in natural killer (NK) cell activation and early B-cell development, however, it is not expressed in most normal mature B-cells. Expression of ZAP-70 has been reported in various lymphomas, including mantle cell lymphoma, small lymphocytic lymphoma and marginal zone lymphoma. During thymocyte development, ZAP-70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP-70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human ZAP70 protein (around aa 247-382) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Human tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background:

Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa

Jurkat cells.Human tonsil or lymph node. EL-4, MOLT-4

Molecular Weight: 70kDa

Gene ID: 7535, 234569

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway