MSK1 antibody

Catalog No. ABIN7127155

The Mouse Monoclonal anti-MSK1 antibody is suitable to detect MSK1 in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for MSK1 antibody (ABIN7127155)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-RPS6KA5-1A8

Product Details anti-MSK1 Antibody

Purpose: RPS6KA5 / MSK1 Antibody

Specificity: The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.

Cross-Reactivity (Details): Human. Predicted to react with Mouse and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human RPS6KA5 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or JAR cells. Ubiquitous nuclear expression. Human prostate, tonsil or brain.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: RPS6KA5

Background:

90 kDa ribosomal protein S6 kinase 5, EC 2.7.11.1, Mitogen and stress activated protein kinase 1, MSPK1, Nuclear Mitogen And Stress Activated Protein Kinase 1, Ribosomal protein S6 kinase 90kD polypeptide 5, Ribosomal Protein S6 Kinase Alpha 5, RLPK, RPS6KA5, RSK Like Protein Kinase, RSKL, S6K alpha 5

HeLa or JAR cells. Ubiquitous nuclear expression. Human prostate, tonsil or brain.

Molecular Weight: ~90kDa

Gene ID: 9252, 510225

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades