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His Tag antibody

WB, ELISA Host: Mouse Monoclonal 3G5H8 unconjugated

Western Blot Positive WB detected in: HIS fusion protein 1 at 20 ng, 10 ng, 5 ng, 2. (His Tag antibody)

Western Blot Positive WB detected in: HIS fusion protein 2 at 20 ng, 10 ng, 5 ng, 2. (His Tag antibody)

2 images

Catalog No. ABIN7131650

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

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Host
85

53

11

6

5

4

1

1
Mouse
Compatible Secondaries
Clonality
86

67

10
Monoclonal
Conjugate
70

22

14

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
131

84

34

25

24

15

14

14

13

13

12

7

6

6

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA

Purification

Protein A purified

Immunogen

HHHHHH(6x His) synthetic peptide conjugate to KLH

Clone

3G5H8

Isotype

IgG2a

Top Product

Discover our top product His Tag Primary Antibody
anti-His Tag antibody
WB, ELISA, IP, IHC Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
Reactivity: Tag, Human WB Host: Mouse Monoclonal 2D5 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 HRP

anti-His Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 Biotin

Application Notes

Recommended dilution: WB:1:500-1:2000,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

His Tag

Alternative Name

6*His (His Tag Products)

Target Type

Tag

Background

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

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