PAK1 antibody (pSer199)

Catalog No. ABIN7138467

The Rabbit Polyclonal anti-PAK1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect PAK1 in samples from Human.

Quick Overview for PAK1 antibody (pSer199) (ABIN7138467)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PAK1 Antibody

Binding Specificity: pSer199

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 199(T-K-S(p)-V-I) derived from Human PAK1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background:

Background:

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Brown J.L., Curr. Biol. 6:598-605(1996).
Sells M.A., Curr. Biol. 7:202-210(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Aliases: ADRB2 antibody, Alpha PAK antibody, Alpha-PAK antibody, MGC130000 antibody, MGC130001 antibody, p21 activated kinase 1 antibody, p21 protein (Cdc42/Rac) activated kinase 1 antibody, p21-activated kinase 1 antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody, p65 PAK antibody, p65-PAK antibody, P68-PAK antibody, PAK alpha antibody, PAK-1 antibody, Pak1 antibody, PAK1_HUMAN antibody, Paka antibody, PAKalpha antibody, Protein kinase MUK2 antibody, Rac/p21-activated kinase antibody, Serine/threonine-protein kinase PAK 1 antibody, STE20 homolog yeast antibody

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis