AKT2 antibody (pSer474)

Catalog No. ABIN7138631

This Rabbit Polyclonal antibody specifically detects AKT2 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for AKT2 antibody (pSer474) (ABIN7138631)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-AKT2 Antibody

Binding Specificity: pSer474

Purpose: Phospho-AKT2 (Ser474) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2

Background:

Background:

General protein kinase capable of phosphorylating several known proteins.

Sun M, et al. (2001) Cancer Res, 61(16): 5985-91.
Yuan ZQ, et al. (2000) Oncogene, 19(19): 2324-30.
Meier R, et al. (1997) J Biol Chem, 272(48): 30491-7.

Aliases: Akt2 antibody, AKT2_HUMAN antibody, HIHGHH antibody, Murine thymoma viral (v-akt) homolog 2 antibody, murine thymoma viral (v-akt) homolog-2 antibody, Oncogene AKT2 protein kinase B beta antibody, PKB antibody, PKB beta antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase Akt-2 antibody, Protein kinase B beta antibody, RAC beta antibody, rac protein kinase beta antibody, RAC-BETA antibody, RAC-beta serine/threonine-protein kinase antibody, RAC-PK-beta antibody, RACbeta antibody, v akt murine thymoma viral oncogene homolog 2 antibody, V-AKT murine thymoma viral oncogene homolog 2 antibody

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling