MAP2K3 antibody (pThr222)

Catalog No. ABIN7138807

This anti-MAP2K3 antibody is a Rabbit Polyclonal antibody detecting MAP2K3 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for MAP2K3 antibody (pThr222) (ABIN7138807)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MAP2K3 Antibody

Binding Specificity: pThr222

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3 .

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MAP2K3

Background:

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

Derijard B., Science 267:682-685(1995).
Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Aliases: AW212142 antibody, dual specificity mitogen activated protein kinase kinase 3 antibody, Dual specificity mitogen-activated protein kinase kinase 3 antibody, MAP kinase kinase 3 antibody, map2k3 antibody, MAPK ERK kinase 3 antibody, MAPK/ERK kinase 3 antibody, MAPKK 3 antibody, MAPKK3 antibody, MEK 3 antibody, MEK3 antibody, Mitogen activated protein kinase kinase 3 antibody, MKK 3 antibody, MKK3 antibody, mMKK3b antibody, MP2K3_HUMAN antibody, PRKMK 3 antibody, PRKMK3 antibody, protein kinase, mitogen-activated, kinase 3 antibody, SAPK kinase 2 antibody, SAPKK 2 antibody, SAPKK2 antibody, Stress activated protein kinase kinase 2 antibody

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2