Aurora A antibody (pThr288)

Catalog No. ABIN7138828

This anti-Aurora A antibody is a Rabbit Polyclonal antibody detecting Aurora A in WB, ELISA and IHC. Suitable for Human.

Quick Overview for Aurora A antibody (pThr288) (ABIN7138828)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Aurora A Antibody

Binding Specificity: pThr288

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background:

Background:

Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
Zhou, H. et al. (1998) Nat Genet 20, 189-93.

Aliases: AIK antibody, ARK-1 antibody, ARK1 antibody, AURA antibody, Aurka antibody, Aurora 2 antibody, Aurora A antibody, Aurora kinase A antibody, Aurora-related kinase 1 antibody, Aurora/IPL1 like kinase antibody, AURORA/IPL1-like kinase antibody, Aurora/IPL1-related kinase 1 antibody, AURORA2 antibody, Breast tumor-amplified kinase antibody, BTAK antibody, hARK1 antibody, IAK antibody, IPL1 related kinase antibody, MGC34538 antibody, OTTHUMP00000031340 antibody, OTTHUMP00000031341 antibody, OTTHUMP00000031342 antibody, OTTHUMP00000031343 antibody, OTTHUMP00000031344 antibody, OTTHUMP00000031345 antibody, OTTHUMP00000166071 antibody, OTTHUMP00000166072 antibody, PPP1R47 antibody, Protein phosphatase 1, regulatory subunit 47 antibody, Serine/threonine kinase 15 antibody, Serine/threonine kinase 6 antibody, Serine/threonine protein kinase 15 antibody, Serine/threonine-protein kinase 15 antibody, Serine/threonine-protein kinase 6 antibody, Serine/threonine-protein kinase aurora-A antibody, STK15 antibody, STK6 antibody, STK6_HUMAN antibody, STK7 antibody

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization