ZNF224 antibody (AA 206-463) (Biotin)

Catalog No. ABIN7145869

Product Details anti-ZNF224 Antibody (Biotin)

Target: ZNF224 (Zinc Finger Protein 224 (ZNF224))

Binding Specificity: AA 206-463

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF224 antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human BUB3-interacting and GLEBS motif-containing protein ZNF207 protein (206-463AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ZNF224

Target: ZNF224 (Zinc Finger Protein 224 (ZNF224))

Alternative Name: ZNF27 (ZNF224 Products)

Synonyms: BMZF-2 antibody, BMZF2 antibody, KOX22 antibody, ZNF255 antibody, ZNF27 antibody, zinc finger protein 224 antibody, ZNF224 antibody

Background:

Background: Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).

Aliases: Zinc finger protein 207 antibody, ZN207_HUMAN antibody, ZNF 207 antibody, ZNF207 antibody

UniProt: O43670