Caspase 4 antibody (AA 82-226) (HRP)

Catalog No. ABIN7146949

This anti-Caspase 4 antibody is a Rabbit Polyclonal antibody detecting Caspase 4 in ELISA. Suitable for Human.

Quick Overview for Caspase 4 antibody (AA 82-226) (HRP) (ABIN7146949)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is conjugated to HRP

Application: ELISA

Product Details anti-Caspase 4 Antibody (HRP)

Binding Specificity: AA 82-226

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Caspase-4 protein (82-226AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: CASP4

Background:

Background: Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion, this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer\'s patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510).

Aliases: Apoptotic cysteine protease Mih1/TX antibody, CASP-4 antibody, CASP4 antibody, CASP4_HUMAN antibody, Caspase 4 apoptosis related cysteine peptidase antibody, Caspase-4 subunit 2 antibody, Caspase4 antibody, ICE(rel)-II antibody, ICE(rel)II antibody, ICEREL II antibody, ICH2 antibody, Mih1/TX antibody, Protease ICH-2 antibody, Protease TX antibody, TX antibody

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity