APEX1 antibody (AA 32-318)

Catalog No. ABIN7150355

Product Details anti-APEX1 Antibody

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Binding Specificity: AA 32-318

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity: Human, Mouse

Purification: >95%, Protein G purified

Immunogen: Recombinant Human DNA-(apurinic or apyrimidinic site) lyase protein (32-318AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 (APEX1 Products)

Synonyms: apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody

Background:

Background: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5\\\'-deoxyribose phosphate and 3\\\'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3\\\'-5\\\' exoribonuclease activity on mismatched deoxyribonucleotides at the 3\\\' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3\\\' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3\\\' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5\\\'-terminal deoxyribose 5\\\'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.

Aliases: AP endonuclease 1 antibody, AP endonuclease class I antibody, AP lyase antibody, APE 1 antibody, APE antibody, APE-1 antibody, APEN antibody, APEX 1 antibody, APEX antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, Apex nuclease 1 antibody, APEX nuclease antibody, APEX1 antibody, APEX1_HUMAN antibody, Apurinic endonuclease antibody, Apurinic-apyrimidinic endonuclease 1 antibody, Apurinic/apyrimidinic (abasic) endonuclease antibody, Apurinic/apyrimidinic endonuclease 1 antibody, Apurinic/apyrimidinic exonuclease antibody, APX antibody, BAP1 antibody, Deoxyribonuclease (apurinic or apyrimidinic) antibody, DNA (apurinic or apyrimidinic site) lyase antibody, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody, EC 4.2.99.18 antibody, HAP 1 antibody, HAP1 antibody, Human Apurinic endonuclease 1 antibody, MGC139790 antibody, Multifunctional DNA repair enzyme antibody, Redox factor 1 antibody, Redox factor-1 antibody, REF 1 antibody, REF 1 protein antibody, REF-1 antibody, REF1 antibody, REF1 protein antibody

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus