Phospholipase C gamma 1 antibody (pTyr775)

Catalog No. ABIN711161

The Rabbit Polyclonal anti-Phospholipase C gamma 1 antibody is suitable to detect Phospholipase C gamma 1 in samples from Human, Mouse and Rat. It has been validated for WB, IF (p) and IHC (p).

Quick Overview for Phospholipase C gamma 1 antibody (pTyr775) (ABIN711161)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: pTyr775

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr775

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: Plc gamma 1

Background:

Synonyms: PLCG1phospho Y775, PLC 1, PLC 148, PLC gamma 1, PLC II, PLC1, PLC148, PLCG 1, PLCG1, PLCgamma1, PLCII, triphosphoinositide phosphodiesterase, 1 phosphatidyl D myo inositol 4 5 bisphosphate, 1 phosphatidylinositol 4 5 bisphosphate, 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphodiesterase gamma 1, phosphoinositidase C, phosphoinositide phospholipase C, Phospholipase C 148, phospholipase C gamma 1 formerly subtype 148, Phospholipase C gamma 1, phospholipase C-148, PLCG1_HUMAN.

Background: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Gene ID: 5335

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling