Tuberin antibody (pSer1798)

Catalog No. ABIN712121

The Rabbit Polyclonal anti-Tuberin antibody has been validated for ELISA, WB, IF (cc), IF (p) and IHC (fro). It is suitable to detect Tuberin in samples from Rat.

Quick Overview for Tuberin antibody (pSer1798) (ABIN712121)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tuberin Antibody

Binding Specificity: pSer1798

Specificity: Phosphorylation site corresponds to Ser1805 in mouse and Ser1800 in rat.

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Tuberin around the phosphorylation site of Ser1798

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: TSC2

Background:

Synonyms: LAM, TSC4, PPP1R160, Tuberin, Tuberous sclerosis 2 protein, TSC2

Background: In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.

Gene ID: 7249

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus