Robo1/ 2 (AA 51-150) antibody

Catalog No. ABIN714851

Product Details

Target: Robo1/ 2

Binding Specificity: AA 51-150

Reactivity: Chicken, Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Cross-Reactivity: Chicken, Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Robo1/2.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Liu, Zhang, Zhang, Zhang, Hao, Song, Li, Feng, Hao, Shen, Min, Yang, Zhao: "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." in: Toxicology letters, Vol. 256, pp. 19-32, (2016) (PubMed).

Target Details

Target: Robo1/ 2

Alternative Name: Robo1/2

Background:

Synonyms: Axon guidance receptor, Deleted in U twenty twenty, DUTT 1, DUTT1, FLJ21882, H Robo 1, H-Robo-1, hRobo 1, Robo 1, Robo1, ROBO1_HUMAN, Roundabout 1, Roundabout axon guidance receptor homolog 1, Roundabout homolog 1, Roundabout homolog1 precurser, Roundabout1, SAX 3, SAX3, Robo 2, Roundabout 2, Roundabout axon guidance receptor homolog 2.

Background: Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior (1). In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively (1). These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway (1). One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals (2,3). Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion (1-3). Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross (1). Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout (2-4). Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray (5,6).

Gene ID: 6091, 6092