SIGLEC8 antibody (AA 17-216)

Catalog No. ABIN7193804

The Mouse Monoclonal anti-SIGLEC8 antibody is suitable to detect SIGLEC8 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for SIGLEC8 antibody (AA 17-216) (ABIN7193804)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 1A5B9

Product Details anti-SIGLEC8 Antibody

Binding Specificity: AA 17-216

Purpose: SIGLEC8 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human SIGLEC8 (AA: 17-216) expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background:

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM, MIM 603492) that mediates an association with SLAM-associated protein (SAP, MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983])

Molecular Weight: 54 kDa

Gene ID: 27181