PDPK1 antibody (pTyr9)

Catalog No. ABIN7223090

This Rabbit Polyclonal antibody specifically detects PDPK1 in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDPK1 antibody (pTyr9) (ABIN7223090)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PDPK1 Antibody

Binding Specificity: pTyr9

Purpose: Rabbit Anti-PDK1 (phospho Tyr9) Polyclonal Antibody

Specificity: Phospho-PDK1 (Y9) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at Y9.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PDK1 Phospho-Tyr9

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: PDPK1, PDK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) is a Protein Coding gene. Among its related pathways are Aldosterone-regulated sodium reabsorption and AMPK signaling pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.

Gene ID: 5170

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals