ARFGAP1 antibody (AA 140-220)

Catalog No. ABIN7223470

The Rabbit Polyclonal anti-ARFGAP1 antibody (ABIN7223470) specifically detects ARFGAP1 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat, Monkey and Cow samples.

Quick Overview for ARFGAP1 antibody (AA 140-220) (ABIN7223470)

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Reactivity: Human, Mouse, Rat, Monkey, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ARFGAP1 Antibody

Binding Specificity: AA 140-220

Purpose: Rabbit Anti-ARF GAP1 Polyclonal Antibody

Specificity: ARF GAP1 Polyclonal Antibody detects endogenous levels of ARF GAP1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human ARF GAP1 at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARFGAP1

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Alternative Name: ARF GAP1

Background: ARFGAP1, ARF1GAP, ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating proteinThe protein encoded by ARFGAP1 (ADP ribosylation factor GTPase activating protein 1) is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

Molecular Weight: 48kD

Gene ID: 55738

UniProt: Q8N6T3

Pathways: p53 Signaling, ER-Nucleus Signaling, Unfolded Protein Response