AKR1C2 antibody (AA 40-120)

Catalog No. ABIN7219996

The Rabbit Polyclonal anti-AKR1C2 antibody is suitable to detect AKR1C2 in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for AKR1C2 antibody (AA 40-120) (ABIN7219996)

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-AKR1C2 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-DD2 Polyclonal Antibody

Specificity: DD2 Polyclonal Antibody detects endogenous levels of DD2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DD2 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKR1C2

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: DD2

Background: AKR1C2, DDH2, Aldo-keto reductase family 1 member C2, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, DD-2, DD2, Dihydrodiol dehydrogenase/bile acid-binding protein, DD/BABP, Trans-1, 2-dihydrobenzene-1,2-diolAKR1C2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. AKR1C2 shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 37kD

Gene ID: 1646

UniProt: P52895

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process