Apoptosis Inhibitor 5 antibody (AA 390-470)

Catalog No. ABIN7213626

The Rabbit Polyclonal anti-Apoptosis Inhibitor 5 antibody is suitable to detect Apoptosis Inhibitor 5 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Apoptosis Inhibitor 5 antibody (AA 390-470) (ABIN7213626)

Target: Apoptosis Inhibitor 5 (API5)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Apoptosis Inhibitor 5 Antibody

Binding Specificity: AA 390-470

Purpose: Rabbit Anti-API5 Polyclonal Antibody

Specificity: API5 Polyclonal Antibody detects endogenous levels of API5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human API5 at AA range: 390-470

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5

Background: API5, MIG8, Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGLAPI5 encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein (apoptosis inhibitor 5) suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 58kD

Gene ID: 8539

UniProt: Q9BZZ5

Pathways: Growth Factor Binding