APEX1 antibody (acLys7)

Catalog No. ABIN7212586

The Rabbit Polyclonal anti-APEX1 antibody (ABIN7212586) specifically detects APEX1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for APEX1 antibody (acLys7) (ABIN7212586)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-APEX1 Antibody

Binding Specificity: acLys7

Purpose: Rabbit Anti-Ref-1 (Acetyl Lys7) Polyclonal Antibody

Specificity: Acetyl-Ref-1 (K7) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K7.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the human Ref-1 around the acetylation site of K7

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: Ref-1

Background: APEX1, APE, APE1, APEX, APX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. APEX1 encodes the major AP endonuclease in human cells. Splice variants have been found for APEX1, all encode the same protein.

Molecular Weight: 35kD

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus