BLK antibody

Catalog No. ABIN7213810

The Rabbit Polyclonal anti-BLK antibody (ABIN7213810) specifically detects BLK in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BLK antibody (ABIN7213810)

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BLK Antibody

Purpose: Rabbit Anti-Blk Polyclonal Antibody

Specificity: Blk Polyclonal Antibody detects endogenous levels of Blk protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Blk around the non-phosphorylation site of Y501

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BLK

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Alternative Name: Blk

Background: BLK, Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-BlkBLK encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein (BLK proto-oncogene, Src family tyrosine kinase) has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Molecular Weight: 58kD

Gene ID: 640

UniProt: P51451

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling